*Fatima Hamza M. baker
College of Health and Medical Techniques/Kufa, Al-Furat Al-Awsat Technical University, 31003 Al-Kufa, Iraq.
ABSTRACT
Background: Gestational diabetes mellitus (GDM) is one of the commonest diseases during pregnancy with significant adverse effect on mother and fetus. Assessment of biochemical parameters like hemoglobin (HG) and random blood glucose (RBG) is essential to understand better the nature of the disease.
Objectives: to compare maternal age, hemoglobin and random blood glucose among women with and without gestational diabetes mellitus.
Materials and Methods: A case-control study design was performed among 100 pregnant women attending Al-Zahraa Teaching Hospital, Najaf, Iraq, during the period from January to June 2026. This study compared 70 pregnant women with gestational diabetes mellitus and 30 pregnant women without gestational diabetes mellitus. The participants’ maternal age, hemoglobin and random blood glucose levels were extracted from the hospital’s medical records. The statistical analysis was achieved by applying the descriptive and inferential statistical tests. The p-value was considered statistically significant at p<0.05.
Results: The mean age of mothers diagnosed with GDM was significantly lower than that of mothers with healthy pregnancy, (28.49 ± 6.89 vs. 31.90 ± 7.70, respectively; P=0.041). Hemoglobin concentration was lower in the GDM group than in the control group (9.90±1.50 vs. 10.35±2.21, respectively). however, the difference was not statistically significant (P = 0.291). At the same time, random blood glucose levels were considerably higher in women diagnosed with GDM than in the control group (263.34 ± 50.93 vs. 107.77±13.56, respectively; P<0.001). The diagnostic accuracy of RBG was found to be highly efficient by ROC analysis, with AUC being 1.000, 100% and 100% for sensitivity and specificity, respectively. Correlation analysis showed that the association between hemoglobin and RBG was weak negative and non- significant (r= -0.139, P=0.168) while showed that the association between maternal age vs. hemoglobin was positive (r= 0.367, P ˂0.001).
Conclusion: the study indicates that proper management of hyperglycemia and anemia may improve pregnancy outcomes and reduce the risk of obstetric and neonatal complications.
KEYWORDS
Gestational diabetes mellitus; Random blood glucose; Hemoglobin; Pregnancy; Biochemical markers.
REFERENCES
1) Sweeting A, Wong J, et al. “A clinical update on gestational diabetes mellitus”. Endocrine Reviews.2022Oct;43(5):763-93. doi:10.1210/endrev /bnac003. doi.org/10.1210/endrev/bnac003.
2) Alejandro EU, Mamerto TP, et al. “Gestational diabetes mellitus: a harbinger of the vicious cycle of diabetes”. International Journal of Molecular Sciences.2020 Jul; 21(14): 5003. doi: 10.3390/ms21145003.
3) Simmons D, Immanuel J, et al. “Treatment of gestational diabetes mellitus diagnosed early in pregnancy”. New England Journal of Medicine.2023 Jun; 388(23): 2132-44. doi: 10.1056/NEJMoa2214956.
4) Apolonia García-Patterson et al.,” Detection and treatment of early gestational diabetes mellitus: a systematic review and meta-analysis of randomized controlled trials”, National Library of Medicine,2025 Nov; 233(5); 380-395. doi: 10.1016/j.ajog.2025.04.059.
5) R Correa-de-Araujo, SS. Yoon, “Clinical outcomes in high-risk pregnancies due to advanced maternal age” J. Womens Health, 30 (2) (2021), pp. 160-167. doi.org/10.1089/jwh.2020.8860.
6) G Swaminathan, A Swaminathan, et al. “Prevalence of gestational diabetes in India by individual socio-economic, demographic, and clinical factors” JAMA Netw Open, 3 (11) (2020). doi:10.1001/jamanetworkopen.2020.25074.
7) M. Lewandowska, “Gestational diabetes mellitus (GDM) risk for declared family history of diabetes, in combination with BMI categories” Int. J. Environ. Res. Public Health, 18 (13) (2021), p. 6936. doi.org/10.3390/ijerph18136936.
8) C. Durnwald, “Gestational diabetes: Linking epidemiology, excessive gestational weight gain, adverse pregnancy outcomes, and future metabolic syndrome”, Semin. Perinatol., 39 (4) (2015), pp. 254-258. doi: 10.1053/j.semperi.2015.05.002.
9) Plows JF, Stanley JL, et al. “The Pathophysiology of Gestational Diabetes Mellitus. International Journal of Molecular Sciences”. 2018;19(11):3342. doi.org/10.3390/ijms19113342.
10) NA ElSayed, RG McCoy, American Diabetes Association Professional Practice Committee. “Diagnosis and Classification of Diabetes: Standards of Care in Diabetes—2025”. Diabetes Care. 2025;48(Suppl 1): S27–S49. doi.org/10.2337/dc25-S002.
11) Metzger BE, Gabbe SG, et al. “International Association of Diabetes and Pregnancy Study Groups Recommendations on the Diagnosis and Classification of Hyperglycemia in Pregnancy”. Diabetes Care. 2010;33(3):676–682. doi.org/10.1053/j.semperi.2015.05.002.
12) McIntyre HD, Catalano P et al. “Gestational diabetes mellitus” Nature Reviews Disease Primers. 2019; 5:47.
13) World Health Organization. “Diagnostic Criteria and Classification of Hyperglycaemia First Detected in Pregnancy”. Geneva: WHO.2014. doi: 10.1016/j.diabres.2013.10.012.
14) Faegheh Firouzi , Fahimeh Ramezani Tehrani et al. “First Trimester Hematological Indices in Gestational Diabetes Mellitus: A Meta-analysis”. Journal of the Endocrine Society. 2025. doi.org/10.1210/jendso/bvaf043.
15) Vida Ghasemi, Mojdeh Banaei et al. “Blood Cells Parameters in Second Trimester of Pregnancy and Gestational Diabetes Mellitus: A Systematic Review and Meta-analysis”. Endocrinology, Diabetes & Metabolism. 2025. doi.org/10.1002/edm2.70024.
16) Vanessa Iribarrem Avena Miranda, Tatiane da Silva Dal Pizzol et al. “Iron Salts, High Levels of Hemoglobin and Ferritin in Pregnancy, and Development of Gestational Diabetes: A Systematic Review. 2022. doi.org/10.1055/s-0042-1755460.
17) GulchekhraIkhtiyarova Akmalovna , Gulrukh Karimova Komilovna et al. “Biochemical, Laboratory and Instrumental Diagnostic Indicators of Early Diagnosis of Women with Gestational Diabetes”. Endocrine and Metabolic Science. 2025. doi.org/10.1016/j.endmts.2025.100252.
18) Yang Y, et al. “First Trimester Hematological Indices in Gestational Diabetes Mellitus: A Systematic Review and Meta-analysis”. Journal of the Endocrine Society. 2025;9(5). doi.org/10.1210/jendso/bvaf043.
19) Vida Ghasemi 1, Mojdeh Banaei et al. “Blood Cell Parameters in the Second Trimester of Pregnancy and Gestational Diabetes Mellitus: A Systematic Review and Meta-analysis”. Endocrinology, Diabetes & Metabolism. 2025. doi:10.1002/edm2.70024.
20) Swinburne A, et al. “First-Trimester Biomarkers of Gestational Diabetes Mellitus: A Scoping Review”. Acta Obstetricia et Gynecologica Scandinavica. 2025. doi.org/10.1111/aogs.70046.
21) Johns EC, Denison FC, et al. “Gestational diabetes mellitus: mechanisms, treatment, and complications”. Trends in Endocrinology & Metabolism. 2018;29(11):743–754. DOI: 10.1016/j.tem.2018.09.004.
22) Liu L, Yan F., et al “Impact of iron supplementation on gestational diabetes mellitus: A literature review. Diabetes, Obesity and Metabolism”. 2023;25(2):342–353. doi.org/10.1111/dom.14886.
23) Fernández-Cao JC, Arija V, et al. “Elevated iron status and risk of gestational diabetes mellitus: A systematic review and meta-analysis”. Acta Diabetologica. 2017. doi.org/10.1111/mcn.12400.
24) Kataria Y, Wu Y, Horskjaer PH, et al. “Iron Status and Gestational Diabetes—A Meta-Analysis”. Nutrients. 2018; 10:621. doi.org/10.3390/nu10050621.
25) Hussein SRM, Abdali AM, et al. “Maternal and neonatal outcomes in pregnancies affected by gestational diabetes mellitus in Najaf, Iraq”. Reproductive Medicine (Central Asia). 2026. doi.org/10.37800/RM.2.2026.765.
26) Li Y, Ren X, et al. “Maternal age and the risk of gestational diabetes mellitus: A systematic review and meta-analysis of over 120 million participants”, Diabetes Research and Clinical Practice. 2020; 162:108044. doi: 10.1016/j.diabres.2020.108044. Article record
27) American Diabetes Association Professional Practice Committee for Diabetes. Diagnosis and Classification of Diabetes: Standards of Care in Diabetes—2026. Diabetes Care. 2026;49(Suppl 1):S27–S49. doi:10.2337/dc26-S002.
28) Sulhariza HZ, Zalilah MS, et al. “Maternal hemoglobin change from early pregnancy to second trimester is associated with risk of gestational diabetes mellitus: a retrospective cohort study”. Frontiers in Nutrition. 2023; 10:1197485. doi:10.3389/fnut.2023.1197485.
29) Liu D, Zhao D, et al. “Associations of maternal hemoglobin and its changes with gestational diabetes mellitus: a prospective cohort study”. Nutrition Journal. 2026; 25:48. doi:10.1186/s12937-026-01306-w.
30) Meek CL, Murphy HR, et al. “Random plasma glucose in early pregnancy is a better predictor of gestational diabetes diagnosis than maternal obesity”. Diabetologia. 2016;59(3):445–452. doi:10.1007/s00125-015-3811-5.
Cite this article
Baker, F. H. M. (2026). Comparative Evaluation of Maternal Age, Hemoglobin, and Random Blood Glucose Levels in Women with and Without Gestational Diabetes Mellitus in Najaf Province, Iraq. INTERNATIONAL JOURNAL OF HEALTH & MEDICAL RESEARCH, 5(9), 929-938. https://doi.org/10.58806/ijhmr.2026.v5i9n11
