ABSTRACT:
This study examines the possible effects of prolonged consumption of soft drinks on sperm parameters in male wistar
rats. Thirty-five (35) male wistar rats were divided into five groups with the control groups administered with distilled water only,
and four other test groups administered with single dose (1.2ml) Coca-Cola, double dose (2.4ml) Coca-Cola (Cocacolax2), 0.6ml
bullet drinks and 1.2ml sugar solution. Aside from the administered samples, food and water were regularly kept at their disposals.
The experimental study lasted for forty (40) days. The weights before and after administration were measured to ascertain the effects
of the test samples on the mean weight difference as well as their mean relative weight difference. Sperm parameters were taken to
ascertain the effects of the test samples on fertility. The results from this study have shown that consumption of soft drinks have
stimulating effects on weights, with corresponding adverse effects on spermatogenesis of test groups when compared with the
control group
KEYWORDS:
Coca Cola, Sperm, Testes, Spermatogenesis, Weight, soft drinks
REFERENCES :
1) Abel L. L., Levy B. B., Brodie B. B., KendalL F. E. (1952). A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem.195(1):357-66.
2) Abreu R.V., Silva-Oliveira, E.M., Moraes, M.F., Pereira, G.S., Moraes-Santos, T. (2011). Chronic coffee and caffeine ingestion effects on the cognitive function and antioxidant system of rat brains. Pharmacology, Biochemistry, and Behavior 99, 659–664.
3) Adham IM, Steding G, Thamm T et al. (2002). The overexpression of the insl3 in female mice causes descent of the ovaries. Mol Endocrinol; 16:244-52.
4) Agarwal A., Ikemoto I. and Loughlin K.R. (1997). Prevention of testicular damage by free radical scavengers. Urology. 50: 759-763.
5) Agbaje IM, Rogers DA, McVicar CM, McClure N, Atkinson AB, et al. (2007) Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod; 22: 1871-7
6) Aitken, R.J., Roman, SD., (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity 1, 15–24.
7) Akdağ MZ, Çelik MS, Ketani A, Nergiz Y, Deniz M, Daşdağ S. (1999). Effect of chronic low-intensity microwave radiation on sperm count, sperm morphology, and testicular and epididymal tissues of rats.
8) Akingbemi BT, Hardy MP. (2001). Oestrogenic and antiandrogenic chemicals in the environment: Effects on male reproductive health. Ann Med; 33:391-403
9) Alkhedaide, A., Soliman, M. M., Salah-Eldin, A.-E., Ismail, T. A., AlshehirI, Z. S., & Attia, H. F. (2016). Chronic effects of soft drink consumption on the health state of Wistar rats: A biochemical, genetic and histopathological study. Molecular Medicine Reports, 13(6), 5109–5117. http://doi.org/10.3892/mmr.2016.51
10) Amaral S, Oliveira PJ, Ramalho-Santos J (2008). Diabetes and the
impairment of reproductive function: possible role of mitochondria and
reactive oxygen species. Curr. Diabetes Rev. 4(1): 46-54.
11) American Medical Association Council on Foods and Nutrition. (1942). Some nutritional aspects of sugar, candy, and sweetened carbonated beverages. JAMA.; 120:763–765.
12) Anderson AM, Jorgensen N, Frydelund-Larsen L et al. (2004); Impaired Leydig cell function in infertile men: A study of 357 idiopathic infertile men and 318 proven fertile controls. J Clin Endocrinol Metab; 89:3161-7.
13) Anderson NL, Anderson NG. (1998). Proteome and proteomics: New technologies, new concepts, and new words. Electrophoresis; 19:1853-1861.
14) Anderson RA, Sharpe RM. (2000). Regulation of inhibin production in the human male and its clinical applications. Int J Androl; 23:136-144.
15) Aquilano DR, Dufau ML. (1985). Cellular localization of rat testicular aromatase activity during development. Endocrinology; 116:38-46.
16) Awoniyi CA, Santulli R, Sprando RL et al. (1989). Restoration of advanced spermatogenic cells in the experimentally regressed rat testis: Quantitative relationship to testosterone concentration within the testis. Endocrinology; 124:1217-1223.
17) Baccetti B, La Marca A, Piomboni P, Capitani S, Bruni E, et al.(2002) Insulin-dependent diabetes in men is associated with hypothalamo-pituitary derangement and with impairment in semen quality. Hum Reprod; 17: 2673-7.
18) Bakos HW, Henshaw RC, Mitchell M, Lane M. (2011); Paternal body mass index is associated with decreased blastocyst development and reduced live birth rates following assisted reproductive technology. Fertil Steril; 95:1700-1704.
19) Barham D. and Trinder P (1972). Improved Color Reagent for the Determination of Blood Glucose by the Oxidase System. Analyst, 97, 142-145.
20) Barnett S.A., (1963). The rat. A Study Behavior. Chicago: Aldine
21) Bassoon R. (2001). Female sexual response: the role of drugs in the management of sexual dysfunction. Obstetrics and gynecology, 98, 350-353.
22) Bay K, Hartung S, Ivell R et al, (2005). Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: Relationship to the luteinizing hormone-testosterone axis. J Clin Endocrinol Metab; 90:3410-8.
23) Behuliak, M., Palffy, R., Gardlik, R., Hodosy, J., Halcak, L., Celec., (2009). Variabilityof thiobarbituric acid reacting substances in saliva. Dis Markers 26, 49–53.
24) Biegel, L.B., Flaws JA., Hirshfield AN., Connor JC., Elliott GS., Ladics GS. et al. (1998). 90-day feeding and one generation reproduction study in Crl: CD BR rats with 17b-estradiol. Toxicol Sci 44, 116–42.
25) Bohring C, Krause E, Habermann B et al. (2001). Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease. Mol Hum Reprod; 7:113-118.
26) Bohring C, Krause W. (2003). Characterization of spermatozoa surface antigens by antisperm antibodies and its influence on acrosome exocytosis. Am J Reprod Immu. 50:411-419.
27) Bremner WJ, Millar MR, Sharpe RM et al, (1994). Immunohistochemically localization of androgen receptors in the rat testis: Evidence for stage-dependent expression and regulation by androgens. Endocrinology; 135:1227-34.
28) Brennan J, Capel B. (2004). One tissue, two fates: Molecular genetic events that underlie testis versus ovary development. Nat Rev Genet; 5(7):509-521.
29) Brooks DE. (1979). Influence of androgens on the weights of the male accessory reproductive organs and on the activities of mitochondrial enzymes in the epididymis of the rat. J Endocrinol 82, 293–303.
30) Bruning JC, Gautam D, Burks DJ, et al, (2000). Role of brain insulin receptor in control of body weight and reproduction. Science.;289:2122– 2125.
31) Buege J.A., Aust S.D. (1978). Microsomal lipid peroxidation. Methods Enzymol 52:302–310.
32) Burbach JP, Luckman SM, Murphy D, Gainer H (2001). Gene regulation in the magnocellular hypothalamoneurohypophysial system. Physiol Rev 81:1197
33) Burkhardt E, Adham IM, Brosig B et al. (1994). Structural organization of the porcine and human genes coding for a Leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3). Genomics; 20:13-19.
34) Caan B, Quesenberry CP Jr, Coates AO. (1998). Differences in fertility associated with caffeinated beverage consumption. Am J Public Health; 88(2):270-4
35) Canadian Council of Animal Care. (1985). Guide to the handling and Use of experimental animals. Ottawa: Ont.; 2 United States NIH publications, no. 85 – 23: 45-47.
36) Carreau S, Levallet J. (1997). Cytochrome P450 aromatase in male germ cells. Folia Histochem Cytobiol; 35:195-202.
37) Carreau S, Bilinska B, Levallet J. (1998). Male germ cells: A new source of estrogens in the mammalian testis. Ann Endocrinol (Paris); 59:79-92.
38) Celec, P., (2012). Intake of cola beverages containing caffeine does not increase, but reduces body weight. European Journal of Clinical Nutrition 66, 538 (author reply 539).
39) Celec, P., Behuliak, M., (2010). Behavioural and endocrine effects of chronic cola intake. Journal of Psychopharmacology 24, 1569–1572.
40) Chalmel F, Rolland AD, Niederhauser-Wiederkehr C et al. (2007). The conserved transcriptome in human and rodent male gametogenesis. Proc Natl Acad Sci USA; 104:8346-8351.
41) Charron M, Wright WW. (2005). Proteases and protease inhibitors. In: Skinner MK, Griswold MD, eds. Sertoli Cell Biology. London: Elsevier Academic Press,:121-152.
42) Com E, Evrard B, Roepstorff P et al. (2003). New insights into the rat Spermatogonia proteome: Identification of 156 additional proteins. Mol Cell Proteomics; 2:248-261.
43) Dasdag S, Akdag MZ, Aksen F, Yilmaz F, Bashan M. (2003). Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes. Bio electromagnetics; 24:182-8. PMID: 12669301
44) Deepinder F, Makker K, Agarwal A. (2007). Cell phones and male infertility: dissecting the relationship. Reprod Biomed Online; 15:266-70. PMID: 17854521
45) de Lamirande, E., Gagnon, C., (1995). Capacitation-associated production of superoxide anion by man spermatozoa. Free Radical Biology and Medicine 18, 487–495.
46) Essader AS, Cargile BJ, Bundy JL et al. (2005). A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in shotgun proteomics. Proteomics; 5:24-34.
47) Eze, N., and Robaire, B. (2012). Androgenic regulation of the structure and function of the epididymis. In: The Epididymis: From Molecules to Clinical Practice (B. Robaire and B. T. Hinton, eds.), pp. 297–316. Kluwer Academic/Plenum Publishers, New York, NY.
48) Fejes I, Závaczki Z, Szöllosi J, Koloszár S, DaruN, Kovács L, Pál A. (2007). Hypothesis: Safety of using mobile phones on male fertility. Arch Androl; 53:105-6. doi: 10.1080/014850
49) Fijak M, Iosub R, Schneider E et al, (2005). Identification of immunodominant autoantigens in rat autoimmune orchitis. J Pathol; 207:127-138.
50) Fritz IB, Tung PS, Ailenberg M. (1993). Proteases and ant proteases in the seminiferous tubules. In: Russell LD, Griswold MD, eds. The Sertoli Cell. Clearwater: Cache River Press,217-235.
51) Gnessi L, Fabbri A, Spera G. (1997). Gonadal peptides as mediators of development and functional control of the testis: An integrated system with hormones and local environment. Endocr Rev; 18:541-609.
52) Grimmond S, Van Hateren N, Siggers P et al. (2000). Sexually dimorphic expression of protease nexin-1 and vanin-1 in the developing mouse gonad prior to overt differentiation suggests a role in mammalian sexual development. Hum Mol Genet; 9(10):1553-1560.
53) Guyot R, Magre S, Leduque P et al, (2003). Differential expression of tissue inhibitor of metalloproteinase type 1 (TIMP-1) during mouse gonad development. Dev Dyn; 227(3):357-366
54) Hong, CY., Shieh, CC., Wu, P., Chiang, BN., (1987). The spermicidal potency of Coca-Cola and Pepsi-Cola. Human Toxicology 6, 395–396.
55) Huang SY, Lin JH, Chen YH et al. (2005). A reference map and identification of porcine testis proteins using 2-DE and MS. Proteomics; 5:4205-4212.
56) Jegou B, Pineau C, Dupaix A. (1999). Paracrine control of testis function. In: Wang C, ed. Male Reproductive Function. Endocrine Update Series. Berlin: Kluwer Academic,41-64.
57) Jégou B. (1993). The Sertoli-germ cell communication network in mammals. Int Rev Cytol; 147:25-96.
58) Josso N, Racine C, Di Clemente N et al., (1998). The role of anti-Müllerian hormone in gonadal development. Mol Cell Endocrinol; 145:3-7.
59) Klenovics, K.S., Boor, P., Somoza, V., Celec, P., Fogliano, V., Sebekova, K., (2013). Advancedglycationend products ininfant formulas donot contributeto insulin resistance associated with their consumption. PloS One 8, e53056.
60) Lebovitz RTM, Johnson L. (1983). Testicular function of rats following exposure to microwave radiation. Bio electromagnetics; 4:107-14. PMID: 6870965
61) Lohmeier TE (2003). Neurohypophysial hormones. Am J Physiol Regul Integr Comp Physiol 285: R715
62) Maes, P., Monakhova, Y.B., Kuballa, T., Reusch, H., Lachenmeier, D.W., (2012). Qualitative and quantitative control of carbonated cola beverages using (1)H NMR spectroscopy. Journal of Agricultural and Food Chemistry 60, 2778–2784.
63) McCusker RR, Goldberger BA, Cone EJ. (2006). Caffeine content of energy drinks, carbonated sodas, and other beverages. J Anal Toxicol.; 30:112–114.
64) McElreavey K, Fellous M. (1999). Sex determination and the Y chromosome. Am J Med Genet; 89(4):176-185.
65) Namekawa SH, Park PJ, Zhang LF et al, (2006). Postmeiotic sex chromatin in the male germline of mice. Curr Biol; 16:660-667.
66) Nassan FL, Priskorn L, Sals-Huetos A, Halldorsson TI, Jensen TK et al, (2021). Association between intake of soft drinks and testicular function in young men. Journal of human Reproduction 36 (12):3036-3048.
67) Ribeiro EP, Rhoden EL, Horn MM, Rhoden C, Lima LP, Toniolo L. (2007). Effects of subchronic exposure to radio frequency from conventional cellular telephone on testicular function in adult rats. J Urol; 177:395-9. PMID: 17162098
68) Rolland AD, Evrard B, Guitton N et al, (1999). Two-dimensional fluorescence difference gel electrophoresis analysis of spermatogenesis in the rat. J Proteome Res 2007; 6:683-697.
69) Russell LD. (1980). Sertoli-germ cell interactions: A review. Gamete Res; 3:179-202.
70) Russell, LD., Malone, JP., and Karpas, SL (1981). Morphologic pattern elicited by agents affecting spermatogenesis by disruption of its hormonal stimulation. Tissue Cell 13, 369–80.
71) Sage C. (2000). An overview of radiofrequency/microwave radiation studies relevant to communication and data. In: Oberfeld G, editor. Proceedings of the International Conference on Cell Tower Sitting Linking Science & Public Health; 7-8 June. Salzburg, Austria. Salzburg: Federal State of Salzburg, Public Health Department; 2000. p. 73-90.
72) Sage C, Carpenter DO. (2009). Public health implications of wireless technologies. Pathophysiology; 16:233-46. doi: 10.1016/j.pathophys.2009.01.011
73) Schlech U, Demougin P, Koch R et al. (2004). Expression profiling of mammalian male meiosis and gametogenesis identifies novel candidate genes for roles in the regulation of fertility. Mol Biol Cell; 15:1031-1043.
74) Shima JE, McLean DJ, McCarrey JR et al. (2004). The murine testicular transcriptome: Characterizing gene expression in the testis during the progression of spermatogenesis. Biol Reprod; 71:319-330.
75) Swain A, Lovell-Badge R. (1999). Mammalian sex determination: A molecular drama. Genes Dev; 13:755-767.
76) Tauler, P., Martinez, S., Moreno, C., Monjo, M., Martinez, P., Aguilo, A., (2013). Effects of caffeine on the inflammatory response induced by a 15-km run competition. Medicine and Science in Sports and Exercise.
77) Töhönen V, Osterlund C, Nordqvist K. (1998). Testatin: A cystatin-related gene expressed during early testis development. Proc Natl Acad Sci USA; 95(24):14208-14213.
78) Umpierre, S.A., Hill, J.A., Anderson, D.J., (1985). Effectof ‘‘Coke’’ on sperm motility. The New England Journal of Medicine 313, 1351
79) Wesensten, N. J. (2014), Legitimacy of concerns about caffeine and energy drink consumption. Nutr Rev, 72: 78–86. doi:10.1111/nure.12146
80) Wong CH, Cheng CY. (2005). The blood-testis barrier: Its biology, regulation, and physiological role in spermatogenesis. Curr Top Dev Biol; 71:263-296.
81) Wu SM, Baxendale V, Chen Y et al, (2004). Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: Biological significance. Genomics; 84:971-981.
82) Yao HH, Tilmann C, Zhao GO et al. (2002). The battle of the sexes: Opposing pathways in sex determination. In: Chadwick D, Goode J, eds. Symposium on the Genetics and Biology of Sex Determination. London: Novartis Foundation:187-198.
83) Vignoli, J.A., Bassoli, D.G., Benassi, M.T., (2011). Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: the influence of processing conditions and raw material. Food Chemistry 124, 863–868.