ABSTRACT:
Background: Plaque is formed by various bacterial microorganisms, one of them is Streptococcus mutans which is
attached to both tooth surface and acrylic plate through polymers between the bacterial matrix and salivary components, which can
cause inflammation of the mucosa, namely denture stomatitis, and angular cheilitiswhich has the potential to cause more serious
problems. complex. Green tea(Camellia sinensis) is herbal plant that is reported to have anti-bacterial activity.
Objective : To determine the effect of immersion in green tea with a concentrationof 20%, green tea with a concentration of 50% and the difference
in concentration between the two on reducing the number of Streptococcus mutans bacteria on heatcured acrylic resin denture plates.
Materials and Methods : This study is an experimental laboratory and uses the pre test and post test method in a control group. The
samples were 27 acrylic specimens measuring 10x10x2mm3 which were divided into 3 working groups, namely distilled water,
brewed green tea with20% content and brewed green tea with 50% content, each of which was 9specimens. Each sample will be
soaked for 15 minutes, the number of colonies is counted and analyzed.
Results: The ANOVA statistical test showed a significant
difference between all treatment groups resulting in a p-value smaller than 0.05.
Conclusion: Green tea infusion with a concentration
of 50% had a significant effectcompared to a concentration of 20% on the number of Streptococcus mutans bacteria on a heat cured
acrylic resin denture base.
KEYWORDS:
Heat cured acrylic resin, Green tea, Antibacterial, Streptococcus mutans
REFERENCES :
1) Rahajoe, S. O. Gigi Tiruan Lengkap Resin Akrilik pada Kasus Full Edentulous. JIKG (Jurnal Ilmu Kedokteran Gigi),
2021;4(2), 53-57
2) Suni, N. A., Wowor, V. N., & Leman, M. A. Uji Daya Hambat Rebusan Daun Pepaya (Carica papaya) terhadap
Pertumbuhan Candida albicans pada Plat Resin Akrilik Polimerisasi Panas. Jurnal e-Gigi. 2017;5(1)
3) Sundari, I., Sofya, P. A., & Hanifa, M. Studi Kekuatan Fleksural antara Resin Akrilik Heat cured dan Termoplastik Nilon
setelah direndam dalam Minuman Kopi Uleekareng (Coffea robusta). Journal of Syiah Kuala Dentistry Society, 2016:1(1),
51-58
4) Silvia, S., Djais, A. A., & Soekanto, S. A. The amount of Streptococcus mutans biofilm on metal, acrylic resin, and valplast
denture bases. Journal of International Dental and Medical Research, 2018:11(3), 899-905
5) Abdillah, M. F. R., Soesetijo, F. A., & Kristiana, D. (2020). Efektivitas Ekstrak Biji Srikaya (Annona squamosa L.) sebagai
Bahan Pembersih GigiTiruan terhadap Daya Hambat Pertumbuhan Streptococcus mutans pada Basis Akrilik Heat Cured.
Pustaka Kesehatan. 2020;8(1), 48-53.
6) Faigenblum, M. J. The denture box. An aid to denture hygiene. British dental journal, 2015;218(1), 9-12.
7) Nugrahini, S. Perubahan Warna pada Plat Gigi Tiruan Resin Akrilik Polimerisasi Panas Setelah Perendaman dalam Larutan
Desinfektan. Soundof Dentistry, 2020;5(1).
8) Choi, Y, & Kang, M. Antibacterial effect of tea tree on Streptococcus mutans. Journal of Korean society of Dental Hygiene,
2017;17(4), 613-620.
9) Rahman, E. F. Efektivitas Ekstrak Daun Dewa (gynura pseudochina (lour.) DC) terhadap Pertumbuhan Candida albicans
pada Plat Dasar Gigi Tiruan Resin Akrilik. 2021;48(123), 32-45.
10) Wijaya, Steven, and Tara Suryantika. Efektivitas Antibakteri Ekstrak Daun Teh Hijau terhadap Bakteri Streptococcus
mutans. Prima Journal of Oral and Dental Sciences 3. 2022: 50-55.
11) Sanders, E.R, Aseptic laboratory techniques: plating methods. JoVE (Journal of Visualized Experiments). 2012 (63),
p.e3064.
12) Widodo, Lestanto Unggul. “Dasar-dasar Praktikum Mikrobiologi.” Microbiological Applications, A Laboratory Manual
in General Microbiology.2017: 1-61
13) Denny, H. and Asep, S, Aktivitas Antioksidan dan Antimikrobial pada Polifenol Teh Hijau. AGROMEDICINE UNILA,
2018:5(2), pp.587-591.
14) Hirasawa, M., Takada, K. and Otake, S, Inhibition of acid production in dental plaque bacteria by green tea catechins. Caries
research. 2006 ;40 (3),pp.265-270
15) Gopal, et.al. Bactericidal Activity of Green tea Extracts: the importance ofcatechin containing nano particles. Scientific
Reports. 2016; 6(1), 1-14.
16) Syah, Andi Nur Alam. Taklukkan Penyakit dengan Teh Hijau. AgroMedia, 2006; p 3-13
17) Anita, A., & Panus, H. Daya Hambat Ekstrak Daun Teh Hijau (Camellia sinensis) terhadap Bakteri Streptococcus mutans.
Jurnal Kesehatan SaintikaMeditory, 2018; 1(1), 1-9.
18) Armidin, R. P., & Yanti, G. N. Effectiveness of rinsing black tea comparedto green tea in decreasing Streptococcus mutans.
Macedonian journal of medical sciences, 2019;7(22), 3799.