ABSTRACT:
Background: Spermicides are substances that can kill spermatozoa. Spermicide circulating in the community contains Nonoxynol9 which, if used for a long time, can irritate the vaginal mucosa and penis. Indonesia has a variety of plants that have the potential to act as anti-fertility, including Sapindus rarak, Centella asiatica, and Azadirachta indica. 

Objective: This study aim to evaluate extract Sapindus rarak, Centella asiatica, and Azadirachta indica. and mixing of third etract to motility and viability of spermatozoa
Guinea pigs.

Methods: The sample of this research was 15 guinea pig spermatozoa, 3-4 months old male guinea pigs taken from the cauda epididymis and vas deferens. Guinea pigs were divided into 5 group treatments with each group treatment incubated for 1, 10, and 30 minutes and repeated each for 5 times. The treatments in this study is control, 200 μl of spermatozoa suspension + 50 μl of Sapindus rarak fruit pulp extract 0.9%; 200 μl of spermatozoa suspension + 50 μl of gotu kola extract 0.9%; 200 μl of
spermatozoa suspension + 50 μl of 0.9% neem seed extract; 200 μl of spermatozoa suspension + 50 μl of the third mixture extract 0.9%. This study used a completely randomized design (CRD). The observations in this study were the motility of guinea pig spermatozoa categories a + b and the viability of guinea pig spermatozoa using a microscope. The data obtained were analyzed statistically using One Way ANOVA. The results of this study indicated that the administration of extract Sapindus rarak, Centella asiatica, and Azadirachta indica and a mixture of the three extracts had a significant effect (P < 0.05) on the motility of guinea pig spermatozoa categories a + b and viability of guinea pig spermatozoa. 

Conclution: This study concludes that the mixture of the three extracts has more potential in reducing the motility of spermatozoa categories a + b and the viability of guinea pig spermatozoa compared to single extracts. The mixture of the three extracts may contribute for reduce quality of sperm.

KEYWORDS:

Lerak Extract (Sapindus rarak), Gotu Kola (Centella asiatica) Extract, Mimba Seed Extract (Azadirachta indica
A.Juss), Spermatozoa guinea pig (Cavia porcellus), Motility, Viability

REFERENCES :

1) BPS. Jumlah Penduduk Indonesia 2019 Mencapai 267 Juta Jiwa |. Dkatadata.co.id. 2019;1.
2) Schreiber CA, Meyn LA, Creinin MD, Barnhart KT, Hillier SL. Effects of long-term use of nonoxynol-9 on vaginal flora. Obstet Gynecol. 2006;107(1):136–43.
3) Xu M, Zhao M, Li RHW, Lin Z, Chung JPW, Li TC, et al. Effects of nonoxynol-9 (N-9) on sperm functions: systematic review and meta-analysis. Reprod Fertil. 2022;3(1):R19–33.
4) Dewanto H. Pengembangan Obat Tradisional Indonesia menjadi Fitofarmaka. Maj Kedokt Indones. 2007;205–11.
5) Umadevi M, Kumar PKS, Bhowmik D, Duraivel S. Journal of Medicinal Plants Studies Medicinal Plants with Potential Antifertility Activity. J Med Plants Stud [Internet]. 2013;1(1):26–33. Available from: www.plantsjournal.com
6) Desai SD, Desai DG, Kaur H. Saponins and their biological activities. Pharma Times. 2009;41(3):13–6.
7) Abu A, Uchendu C, Ofukwu R. Sperm immobilization properties of aqueous ethanolic extract of Hymenocardia acida stem bark. Maced J Med Sci. 2011;4(3):261–4.
8) Sihombing W, Akmal M, Wahyuni S, Nasution I, – R, – H. EFEK EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PERKEMBANGAN SEL SPERMATID TIKUS (Rattus norvegicus). J Med Vet. 2015;9(1):71–6.
9) Solihati N, Purwantara B, Supriatna I, Winarto A. Histomorphological changes, sperm quality and testosterone level after administration and cessation of Pegagan (Centella asiatica) extract in rats. J Indones Trop Anim Agric. 2018;43(3):218– 29.
10) Ashfahani ED, Wiratmini NI, Sukmaningsih AASA. Motilitas Dan Viabilitas Spermatozoa Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Temu Putih (Curcuma zedoaria (Berg.) Roscoe.). J Biol. 2007;1(1):20–3.
11) Roop JK, Dhaliwal PK, Guraya SS. Extracts of Azadirachta indica and Melia azedarach seeds inhibit folliculogenesis in albino rats. Brazilian J Med Biol Res. 2005;38(6):943–7.
12) Auta T, Hassan AT. Reproductive toxicity of aqueous wood-ash extract of Azadirachta indica (neem) on male albino mice. Asian Pacific J Reprod [Internet]. 2016;5(2):111–5. Available from: 

http://dx.doi.org/10.1016/j.apjr.2016.01.005
13) Asif M. A Review on Spermicidal Activities of Azadirachta indica. Online. 2013;1(5):62–80.
14) Science E. Neem leaves extract ( Azadirachta indica A . Juss ) on male reproductive system : a mini-review Neem leaves extract ( Azadirachta indica A . Juss ) on male reproductive system : a mini-review. 2019;
15) Dubey R, Dubey K. Sperm Immobilization Potential of Saponin Extract of Ziziphus mauritiana. J Drug Deliv Ther. 2019;9:78–80.
16) Nurliani A, Santoso HB. EFEK SPERMATISIDA EKSTRAK KULIT KAYU DURIAN (Durio zibethinus Murr) TERHADAP MOTILITAS DAN KECEPATAN GERAK SPERMATOZOA MANUSIA SECARA IN VITRO. J Sains
dan Terap Kim [Internet]. 2016;4(1):72–9. Available from:
http://ppjp.unlam.ac.id/journal/index.php/jstk/article/view/2051
17) Susetyarini E, Wahyono P, Latifa R, Nurrohman E. New Zealand rabbit ’ s spermatozoa after the treatment of tannin extraction of Pluchea indica. Bioedukasi J Biol dan Pembelajarannya. 2020;18(2):59–68.
18) Yuan J, Yang D, Liang Y, Gao W, Ren Z, Zeng W, et al. Alkaloids from Areca (Betel) Nuts and Their Effects on Human Sperm Motility In Vitro. J Food Sci. 2012;77(4):70–8.
19) Yakubu MT. Effect of a 60-day oral gavage of a crude alkaloid extract from Chromolaena odorata leaves on hormonal and spermatogenic indices of male rats. J Androl. 2012;33(6):1199–207.
20) Lestari SW, Larasati MD, Mansur IG, Margiana R. Sperm Na+ K+-ATPase and Ca2+-ATPase activities: A potential predictive parameter of sperm motility disorder in infertile men. Biomed Pharmacol J. 2018;11(1):411–6.
21) Boulais M, Soudant P, Le Goïc N, Quéré C, Boudry P, Suquet M. ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster (Crassostrea gigas). Aquaculture [Internet]. 2017;479(May):114–9. Available from: http://dx.doi.org/10.1016/j.aquaculture.2017.05.035
22) Sabés-Alsina M, Planell N, Gil S, Tallo-Parra O, Maya-Soriano MJ, Taberner E, et al. Metabolic activity of sperm cells: Correlation with sperm cell concentration, viability and motility in the rabbit. Zygote. 2016;24(5):707–13.
23) Amaral A, Lourenço B, Marques M, Ramalho-Santos J. Mitochondria functionality and sperm quality. Reproduction. 2013;146(5).
24) Amilah S, Sukarjati S, Rachmatin DP, Masruroh M. Leaf and Petiole Extract of Centella Asiatica are Potential for Antifertility and Antimicrobial Material. Folia Medica Indones. 2019;55(3):188.
25) Lu Z, Wang L, Zhou R, Qiu Y, Yang L, Zhang C, et al. Evaluation of the spermicidal and contraceptive activity of Platycodin D, a saponin from Platycodon grandiflorum. PLoS One. 2013;8(11):1–11.
26) Biologi J, Viability ON. MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT ( Mus musculus L .) SETELAH PEMBERIAN EKSTRAK TEMU PUTIH ( Curcuma zedoaria ( Berg .) Roscoe .) ON VIABILITY AND MOTILITY OF MICE ( Mus musculus L .) SPERMATOZOA. 2007;(1):20–3.
27) Vishalakshi Devi D, Urooj A. Nutrient profile and antioxidant components of Costus speciosus Sm. and Costus igneus Nak. Indian J Nat Prod Resour. 2010;1(1):116–8.